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Showing: 2801 - 2850 of 4184 results

  2. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  4. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101880-plasmid-map-sequence-id-194708

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  6. pJK605

    Plasmid
    #72530
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72530-plasmid-map-sequence-id-141993

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  7. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

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  8. pJK289

    Plasmid
    #71553
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine (please see deposito…
    Promoter
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71553-plasmid-map-sequence-id-139945

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  9. pJK501

    Plasmid
    #72246
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gln206 to Arg and changes tryptophan-151 to pheny…
    Promoter
    T7 and endogenous
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72246-plasmid-map-sequence-id-139355

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  11. pJK304

    Plasmid
    #72225
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72225-plasmid-map-sequence-id-134624

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  12. pJK303

    Plasmid
    #72224
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72224-plasmid-map-sequence-id-134623

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  13. pJK579

    Plasmid
    #72426
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    4 silent mutations introduce unique internal SpeI…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72426-plasmid-map-sequence-id-133996

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  14. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

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  15. pJK600

    Plasmid
    #71605
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71605-plasmid-map-sequence-id-131682

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  16. 2GSF

    Plasmid
    #51363
    Depositor
    Cheryl Arrowsmith
    Insert
    2GSF (EPHA3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. GIMAP1 (3LXW)

    Plasmid
    #25336
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GIMAP1:E25-R253 (GIMAP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids E25-R253
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. GIMAP2

    Plasmid
    #28166
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GTPase IMAP family member 2 (GIMAP2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    AIG1 domain only
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. HOXD1 (murine, partial) HIS-tag pET

    Plasmid
    #8560
    Depositor
    Corey Largman
    Insert
    HOXD1 (Hoxd1 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Partial protein containing 10 AA N-terminal to HD…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. pNGFP-BC

    Plasmid
    #161956
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161956-plasmid-map-sequence-id-319761

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  21. pCGFP-BC

    Plasmid
    #161959
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161959-plasmid-map-sequence-id-319762

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  25. SPYtag-Histag-Avidin

    Plasmid
    #214836
    Purpose
    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-Histag-Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214836-plasmid-map-sequence-id-423115

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  26. Non tagged Avidin

    Plasmid
    #214835
    Purpose
    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Non tagged Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214835-plasmid-map-sequence-id-423116

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  32. WDR5 (2H9N)

    Plasmid
    #25229
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. 5VC9 (CXXC4)

    Plasmid
    #101263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC4 (CXXC4 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101263-plasmid-map-sequence-id-197652

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  46. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. 6BYB (L3MBTL1)

    Plasmid
    #119957
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119957-plasmid-map-sequence-id-232627

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  50. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV420H2 (KMT5C Human)
    Use
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2801 - 2850 of 4184 results