We narrowed to 24,019 results for: Nov

1. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

   Plasmid

   #191331

   Purpose

   Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-CBX1mut-Dronpa

   Plasmid

   #138250

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-Dronpa
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK0_004

   Plasmid

   #123961

   Purpose

   Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   cRosa26 destination
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ARB287d

   Plasmid

   #124018

   Purpose

   Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ARB287e

   Plasmid

   #124019

   Purpose

   Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JPF0464

   Plasmid

   #124035

   Purpose

   Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR_pEF1a-iRFP713_non targeting sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JPF0465

   Plasmid

   #124036

   Purpose

   Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR_pEF1a-iRFP713_mScarlet sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JPF0466

    Plasmid

    #124037

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_tagBFP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JPF0468

    Plasmid

    #124038

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK234_005

    Plasmid

    #123992

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA homology arm-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MTK234_006

    Plasmid

    #123993

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTK234_007

    Plasmid

    #123994

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ARB287a

    Plasmid

    #124015

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARB287b

    Plasmid

    #124016

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ARB287c

    Plasmid

    #124017

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-rglpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK234_054

    Plasmid

    #123959

    Purpose

    Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    CXCR4 sa sgRNA 3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK0_011

    Plasmid

    #123966

    Purpose

    Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hRosa26-PhiC31 landing pad
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK0_028

    Plasmid

    #123968

    Purpose

    Encodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    cRosa26 landing pad destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge