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pET21 E2F1

Plasmid

#90313

Purpose

Expresses human E2F1 in bacteria

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 (E2F1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pSprayer

Plasmid

#165148

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNB655

Plasmid

#60142

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBait-YY1

Plasmid

#165147

Purpose

Bait plasmid for PROBER with YY1-binding site (positive control)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

3X YY1 motif

Use

Unspecified

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_FEV

Plasmid

#167757

Purpose

Expresses FEV in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

FEV (FEV Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF3

Plasmid

#187740

Purpose

MAC-tagged gene expression of human ELF3

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF3 (ELF3 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EGR-1 TurboRFP

Plasmid

#52306

Purpose

Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Varma et al Lab Chip. 2015 Feb 4.

Insert

EGR-1 Promoter (Egr1 Mouse)

Tags

TurboRFP

Expression

Mammalian

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7_hsa-miR-34a

Plasmid

#25791

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a (TP53 Human)

Use

Lentiviral and RNAi

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pETM33_SUFU_Sufu

Plasmid

#178488

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SUFU_Sufu (SUFU Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF2

Plasmid

#187737

Purpose

MAC-tagged gene expression of human ELF2

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF2 (ELF2 Human)

Expression

Mammalian

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_TEAD2

Plasmid

#167804

Purpose

Expresses TEAD2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TEAD2 (TEAD2 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELK4

Plasmid

#187745

Purpose

MAC-tagged gene expression of human ELK4

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELK4 (ELK4 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_ETV6

Plasmid

#167755

Purpose

Expresses ETV6 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETV6 (EN2 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PAGFP_NS2B3

Plasmid

#195755

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Depositor

Partha Mondal

Article

Varghese et al bioRxiv 2022.12.18.520514

Insert

Dengue (Type-2) NS2B3

Tags

PA-GFP

Expression

Mammalian

Mutation

No mutations

Promoter

CMV

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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