We narrowed to 146,063 results for: ins;

1. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST47-Arl13b12

   Plasmid

   #40872

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   no stop codon

   Promoter

   CMV

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD287 (mKate2^SEC^3xMyc

   Plasmid

   #70685

   Purpose

   mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

   Plasmid

   #133802

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA5
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAST td tomato Rab5 WT

   Plasmid

   #58446

   Purpose

   expression of drosophila td tomato Rab5

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   td tomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

   Plasmid

   #132545

   Purpose

   Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv d0

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #213034

    Purpose

    fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL_dstβL

    Plasmid

    #73805

    Purpose

    Plasmid for selection for insertion and homodimerization

    Depositor

    Sarel Fleishman

    Article

    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

    Inserts

    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    

    Use

    Tags

    Part Of the dstbl chiemra, Part of the dsTbL chim…

    Expression

    Bacterial

    Mutation

    Promoter

    NaN and ToxR promotor

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST40-Arl13b11

    Plasmid

    #40873

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

14. p40phox-PX (1-144)

    Plasmid

    #119123

    Purpose

    Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p40phox-PX (1-144)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-Arl13b2

    Plasmid

    #40871

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pDEST40-Arl13b89

    Plasmid

    #40874

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC-STITCH

    Plasmid

    #129535

    Purpose

    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    STITCH
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p47phox-PX (1-122)

    Plasmid

    #119124

    Purpose

    Bacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p47phox-PX (1-122)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASP dsRed Rab11 WT

    Plasmid

    #44829

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. mPLD1-PX (71-210)

    Plasmid

    #119127

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD1-PX (71-210)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge