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We narrowed to 62,951 results for: cloning

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Showing: 11381 - 11400 of 62951 results

  2. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  3. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    63196-plasmid-map-sequence-id-104896

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  4. pAV113

    Plasmid
    #63180
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63180-plasmid-map-sequence-id-104851

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  5. pMuLE ENTR 7SK-miR-30 L1-L4

    Plasmid
    #62180
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid
    #62127
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62127-plasmid-map-sequence-id-112700

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  7. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid
    #62131
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62131-plasmid-map-sequence-id-112709

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  12. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid
    #176277
    Purpose
    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Martin Riccomagno
    Publication
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    MCS-P2A-mCherry
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    human Synapsin I
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176277-plasmid-map-sequence-id-350686

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  14. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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  18. pHW2173

    Plasmid
    #255981
    Purpose
    N-terminal TAG-IN destination vector with SEC-LoxP for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD363 with LoxP)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255981-plasmid-map-sequence-id-512522

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  19. pHW2174

    Plasmid
    #255982
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox2272 for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255982-plasmid-map-sequence-id-511513

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  20. pHW2175

    Plasmid
    #255983
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox511I for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD364 with Lox511I)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255983-plasmid-map-sequence-id-511495

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Showing: 11381 - 11400 of 62951 results