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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Expression

Plant

Promoter

At U6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG082

Plasmid

#114000

Purpose

Level 0 module. NifH promoter for nodule-specific expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

NifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHES945

Plasmid

#112643

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

CRY2PHR-GAL4DBD

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 100-175

Plasmid

#99667

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (101-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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