We narrowed to 18,078 results for: comp

1. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203219

   Purpose

   Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-QNZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_SBP-EGFP-ColX

   Plasmid

   #222305

   Purpose

   Synchronize the trafficking of CollagenX from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   signal peptide of COL10A1 removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_SBP-EGFP-gp135

   Plasmid

   #222306

   Purpose

   Synchronize the trafficking of gp135/podocalyxin from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   signal peptide removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_SBP-EGFP-LAP

   Plasmid

   #222322

   Purpose

   Synchronize the trafficking of LAP from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Str-KDEL_SBP-EGFP-EGFR

   Plasmid

   #222325

   Purpose

   Synchronize the trafficking of EGFR from the ER.

   Depositor

   Franck Perez

   Article

   Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

   Insert

   Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

   Plasmid

   #203965

   Purpose

   Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

   Depositor

   Elaine Fuchs

   Article

   Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

   Insert

   ARID1A (Arid1a Mouse)
   

   Use

   Sleeping beauty transposon

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-F-box:gs10:FRB:HA

   Plasmid

   #228531

   Purpose

   F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   F-box:gs10:FRB:HA (SKP2 Synthetic, Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

   Plasmid

   #228534

   Purpose

   Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Rbx1:gs10:FRB:HA (RBX1 Synthetic, Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

   Plasmid

   #203443

   Purpose

   Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM148: pAAV.CMV-Cas13e-NT sgRNA

    Plasmid

    #203446

    Purpose

    Plasmid expressing active RfxCas13d with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

    Plasmid

    #203447

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

    Plasmid

    #203448

    Purpose

    Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA array
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-actin

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 CHMP4A 153-222

    Plasmid

    #108256

    Purpose

    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4A residues 153-222 (CHMP4A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 CHMP6 145-201

    Plasmid

    #104597

    Purpose

    Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP6 residues 145-201 (CHMP6 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CHMP7 366-453

    Plasmid

    #104598

    Purpose

    Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP7 residues 366-453 (CHMP7 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

    Plasmid

    #215910

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332D, S1346D, S1616D, S169…

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge