We narrowed to 30,936 results for: LIS;

1. NMD2_lenti

   Plasmid

   #216221

   Purpose

   Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_b-globin intron x2
   

   Expression

   Mammalian

   Available Since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE491

   Plasmid

   #149292

   Purpose

   T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEE531

   Plasmid

   #149290

   Purpose

   T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Nd1

   Plasmid

   #191523

   Purpose

   Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Tags

   1D4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

   Plasmid

   #192406

   Purpose

   To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::Turbo GFP
   

   Use

   Synthetic Biology

   Tags

   N7

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRII-Topo Plexin D1 in situ probe

   Plasmid

   #45621

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

   Insert

   Plexin D1 in situ probe (Plxnd1 Mouse)
   

   Use

   In situ

   Mutation

   fragment contains bp# 5751-6406 of NM_026376.3

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

10. 6xHis-TEV-UBC-9

    Plasmid

    #87055

    Purpose

    Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    UBC-9 (ubc-9 Nematode)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. KTK_251

    Plasmid

    #180546

    Purpose

    Entry vector containing mScarlet

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    mScarlet
    

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

    Plasmid

    #192380

    Purpose

    To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6M-Pak1 P13A

    Plasmid

    #12207

    Depositor

    Jonathan Chernoff

    Article

    Galisteo et al J Biol Chem. 1996 Aug 30. 271(35):20997-

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P13A: disrupts first polyproline region of Pak1

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. KTK_136

    Plasmid

    #180520

    Purpose

    Empty backbone for D1.1 cloning, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CaMaKIIalpha-mKO2

    Plasmid

    #86156

    Purpose

    Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2016 Oct 11;17(3):720-734. doi

    Insert

    mKO2
    

    Use

    Lentiviral

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pERB257

    Plasmid

    #67761

    Purpose

    Expresses KLC1-mCherry-eDHFR

    Depositor

    Michael Lampson

    Article

    Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

    Insert

    KLC1-mCherry-eDHFR (Klc1 Rat)
    

    Tags

    eDHFR and mCherry

    Expression

    Mammalian

    Mutation

    contains Klc1 amino acids 1-175

    Promoter

    CAG

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRII-Topo Ptn in situ probe

    Plasmid

    #45632

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Ptn in situ probe (Ptn Mouse)
    

    Use

    In situ

    Mutation

    fragment contains bp# 1241-1830 of NM_008973

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

20. KTK_001

    Plasmid

    #180517

    Purpose

    Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge