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pJK_proNR2_LGK.9

Plasmid

#81162

Purpose

Expresses levoglucosan kinase variant LGK.9 with promoter proNR2

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase

Tags

His6x

Expression

Bacterial

Mutation

LGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…

Promoter

proNR2

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
YCp33-YDL183c-HA

Plasmid

#70164

Purpose

yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

3xHA

Expression

Yeast

Promoter

YDL183c (217 nucleotides upstream of the ATG)

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
pDGB1_omega2R

Plasmid

#68237

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCVD531

Plasmid

#66160

Purpose

A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned into the HindIII site of pBR322.

Depositor

James Kaper

Article

Nishibuchi et al FEMS Microbiol Lett. 1990 Feb;55(3):251-

Insert

tdh-like

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-20nmSAH-GFP(1-10)

Plasmid

#61661

Purpose

20nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-20nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-111

Plasmid

#61824

Purpose

NpuDnaB(delta283)dN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaB(delta283)dN11 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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