161,152 results

1. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130999

   Purpose

   Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_Gal4UAS_IRES_mC_pGK_tBFP

   Plasmid

   #79123

   Purpose

   Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Rab4B

   Plasmid

   #61801

   Purpose

   Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab4B (RAB4B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15TV-L-hlPYK WT

   Plasmid

   #187975

   Purpose

   Expresses the human liver pyruvate kinase

   Depositor

   Audrey Lamb

   Article

   McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

   Insert

   hlPYK WT
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-tetOn-IRES-G418 (EtO)

   Plasmid

   #84776

   Purpose

   Second generation lenti tetracycline operator (on)

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Insert

   TET OPERATOR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTIGHT

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgLenti-orthogonal

   Plasmid

   #105997

   Purpose

   Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; S.pyogenes sgrna expressio…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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