162,981 results

1. PDIA4-bio-His

   Plasmid

   #52071

   Purpose

   Expresses full-length Protein disulfide-isomerase A4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PDIA4 (PDIA4 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro-cGASsh4

   Plasmid

   #127646

   Purpose

   Knock-down of human cGAS

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   cGAS shRNA (CGAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30b-7+-SrtA

   Plasmid

   #105602

   Purpose

   expressed 7+ sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7+ mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

   Plasmid

   #189627

   Purpose

   Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   hM3D(Gq)
   d2tTA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn and pRAM

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOG130

   Plasmid

   #105336

   Purpose

   Gateway entry clone generation

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_WNV_PrME

   Plasmid

   #207313

   Purpose

   Mammalian expression of West Nile Virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   WNV_PrME (POLY West Nile Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTEF-Sup35C

   Plasmid

   #1202

   Depositor

   Susan Lindquist

   Article

   True et al Nature 2004 Sep 9;431(7005):184-7. Epub

   Insert

   Sup35 C-terminus (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Sup35 C-terminus ~254aa-end

   Promoter

   TEF

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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