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TRE:mCherry-p2a- bReaChES

Plasmid

#163037

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

mCherry-p2a-bReaChES

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSEM238

Plasmid

#161515

Purpose

Neuronal expression of histamine-sensitive chloride channel for negative selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Psnt-1 | histamine | rpl-3 UTR]

Expression

Worm

Promoter

Psnt-1

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JD639

Plasmid

#160398

Purpose

Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

Depositor

Jorge Dubcovsky

Publication

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis miR396-resistant GRF4-GIF1 chimera

Use

Binary vector

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-mGreenLantern

Plasmid

#161913

Purpose

mGreenLantern fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

6xHis

Expression

Bacterial

Promoter

araB

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMK262 (RAD21-mAC Neo)

Plasmid

#140538

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCherry

Expression

Mammalian

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Publication

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Publication

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Publication

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Publication

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Publication

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH2B-emiRFP703

Plasmid

#136567

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP703

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAVS1-TRE-MA4-GFP

Plasmid

#114380

Purpose

Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

Depositor

Yuet Wai Kan

Publication

Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

MLL-AF4 fusion gene (AFF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

TRE

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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M3-M14-dCas9

Plasmid

#134779

Purpose

Expression of fusion protein M3-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 S. pyogenes, Human)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria1-GFP KI

Plasmid

#131489

Purpose

Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 acGFP

Plasmid

#128047

Purpose

Mammalian expression vector with N terminal AcGFP

Depositor

Oskar Laur

Type

Empty backbone

Tags

AcGFP

Expression

Mammalian

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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scOrange chromoprotein

Plasmid

#117840

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, scOrange

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor_AcCAST_kanR

Plasmid

#127925

Purpose

Kanamycin resistance donor for AcCAST

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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