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pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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CDCA5 C9.4 gRNA

Plasmid

#90600

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDCA5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDCA5 (Guide Designation C9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
PPP2R2C B7.5 gRNA

Plasmid

#90849

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2C (Guide Designation B7.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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HDGF A7.3 gRNA

Plasmid

#90708

Purpose

3rd generation lentiviral gRNA plasmid targeting human HDGF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HDGF (Guide Designation A7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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HDGF A8.3 gRNA

Plasmid

#90709

Purpose

3rd generation lentiviral gRNA plasmid targeting human HDGF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HDGF (Guide Designation A8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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NXT1 A5.3 gRNA

Plasmid

#90814

Purpose

3rd generation lentiviral gRNA plasmid targeting human NXT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NXT1 (Guide Designation A5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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Unc5c.y-Fc-His

Plasmid

#72181

Purpose

Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.y (Unc5c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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KIF11 F6.3 gRNA

Plasmid

#90717

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F6.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TOP2A G12.2 gRNA

Plasmid

#90915

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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NDC80 F12.1 gRNA

Plasmid

#90787

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F12.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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NDC80 F11.1 gRNA

Plasmid

#90786

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H1.1 gRNA

Plasmid

#90886

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H2.1 gRNA

Plasmid

#90887

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3e(S)-AP-His

Plasmid

#72020

Purpose

Expresses the Sema3E protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.y-AP-His

Plasmid

#72055

Purpose

Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.y (Unc5c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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