We narrowed to 21,328 results for: KIN

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pGST TCF4 DBD

Plasmid

#16488

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

TCF4 DNA binding domain (TCF7L2 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 265-496

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
MAC_N_LHX2

Plasmid

#167776

Purpose

Expresses LHX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

LHX2 (LHX2 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP-3x-miR708-5p-TS

Plasmid

#117381

Purpose

An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase like 1 (HMGCLL1)

Plasmid

#52434

Purpose

mammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)

Depositor

Henry Miziorko

Article

Montgomery et al J Biol Chem. 2012 Sep 28;287(40):33227-3

Insert

HMGCLL1 (HMGCLL1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
MBP-GTSE1-His6

Plasmid

#69109

Purpose

For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Tags

His6 and MBP

Expression

Bacterial

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse, Human)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 48-232 (#182)

Plasmid

#99920

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 48-232

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFAB3737

Plasmid

#47838

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 7 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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MBC1-PuroIII

Plasmid

#153419

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Article

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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HSC1-D4Z4-GiP

Plasmid

#58542

Purpose

Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-SP110

Plasmid

#178448

Purpose

doxycycline-inducible expression of Human SP110 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SP110 (SP110 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-L166P/K130R

Plasmid

#29349

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Leu166Pro and Lys130Arg

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/GS-DJ1-L166P-V5

Plasmid

#29343

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

L166P

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only
pTA231-p.Syn-Dendra2Hfx

Plasmid

#164660

Purpose

expression of H. volcanii codon-optimized Dendra2

Depositor

Anita Marchfelder

Article

Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

Insert

Dendra2Hfx

Use

Archaeal expression

Mutation

codon-optimization for Haloferax volcanii

Promoter

p.Syn

Available Since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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