We narrowed to 14,381 results for: cas9 genes

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

GPDp

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T_mouseH3.1-HaloTag

Plasmid

#244242

Purpose

CRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

H3.1 (H3c1 Mouse)

Use

CRISPR

Tags

HaloTag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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ACBE-NW

Plasmid

#240624

Purpose

A-to-C base editor with narrowed editing window

Depositor

Tingting Jiang

Article

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Insert

TadA8e(N108Q, M151C, Q154Y)-nCas9 (D10A)-mAAG

Use

CRISPR

Expression

Mammalian

Mutation

TadA8e(N108Q, M151C, Q154Y),Cas9 (D10A),mAAG (R16…

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgROSA26

Plasmid

#231562

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgROSA26

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pODN-AAVS1-mNG-CAAX

Plasmid

#183870

Purpose

Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-CAAX

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRDB_118

Plasmid

#216089

Purpose

Cas9 [Sp] CRISPRa targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_836

Plasmid

#216088

Purpose

Cas9 [Sp] knockout targeting CD55, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD55 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pODN-H2BC11-mR2

Plasmid

#183866

Purpose

Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)

Use

CRISPR; Donor template

Tags

linker-mRuby2

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-mR2-MYH10

Plasmid

#183869

Purpose

Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 homology arms with mRuby2-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mRuby2-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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MTK0_003

Plasmid

#123925

Purpose

Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Cas9-sgRNA destination

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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SunTagCACTA1g2-22aa-TET1cd

Plasmid

#106437

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pKS7107

Plasmid

#89051

Purpose

Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Nm crRNA
Nm tracrRNA
hNmCas9

Use

CRISPR

Tags

HA tag, NLS, and SV40 NLS

Promoter

EF1a and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_2

Plasmid

#104049

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_1

Plasmid

#104048

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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