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pEB2-mNeptune2

Plasmid

#104012

Purpose

Plasmid encoding mNeptune2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mNeptune2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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J23103_GB

Plasmid

#65991

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23107_GB

Plasmid

#65999

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23116_GB

Plasmid

#66003

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNC1136

Plasmid

#41562

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAT206/Sept7shRNA

Plasmid

#38299

Depositor

Matthew Krummel

Article

Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

Insert

Septin 7 (Septin7 Mouse)

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Tags

His tag with TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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CoV-NSP3-c001

Plasmid

#173082

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep

Tags

mCerulean

Expression

Bacterial

Mutation

E1000-K1173, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c003

Plasmid

#173105

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c005

Plasmid

#173106

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Tags

Nanoluc

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEB2-mRFP1*

Plasmid

#104000

Purpose

Plasmid encoding mRFP1*

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRFP1*

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLAVIKEF...T…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-EGFP

Plasmid

#102782

Purpose

Baculovirus rescue vector, VSVGED pseudotype, eGFP

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

EGFP

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pQnl_Pcphy23

Plasmid

#191354

Purpose

Clostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoter

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Pcphy23-NanoLuc

Expression

Bacterial

Promoter

Pcphy23

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2-mRFP1

Plasmid

#104001

Purpose

Plasmid encoding mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRFP1

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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KG#65

Plasmid

#110933

Purpose

Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

unc-17 beta promoter
unc-54 3' control region

Expression

Bacterial

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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THI VP64 pGAP T-

Plasmid

#161487

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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