We narrowed to 2,378 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pFH49

   Plasmid

   #128211

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHREAC

   Plasmid

   #134908

   Purpose

   Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   double 35S

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GAL-Aur1-2GFP-Hygromycin

   Plasmid

   #184775

   Purpose

   Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   proGAL-Aur1 (AUR1 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-BRAT1

   Plasmid

   #62802

   Purpose

   Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

   Depositor

   Toru Ouchi

   Article

   So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

   Insert

   BRAT1 (BRAT1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-neo-Strep_Flag_Cterm

   Plasmid

   #102645

   Purpose

   Epitope tag c-terminus with SBP-Flag moiety

   Depositor

   Michael E. Wright

   Article

   Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

   Type

   Empty backbone

   Tags

   SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

   Expression

   Mammalian

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-VPS28

   Plasmid

   #34803

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   vacuolar protein sorting 28 (yeast) (VPS28 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-FLAG

   Plasmid

   #183511

   Purpose

   Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Type

   Empty backbone

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pRPR1(TetO)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrEno

    Plasmid

    #115474

    Purpose

    Eno-promoter driven expression vector for protein production in Trichoderma reesei

    Depositor

    Steve Decker

    Article

    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

    Insert

    Cel7A
    

    Use

    Unspecified

    Mutation

    Introns have been removed

    Promoter

    Trichoderma reesei Enolase (Eno) promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRU295

    Plasmid

    #167694

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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