We narrowed to 31,888 results for: Eng

1. pDL52 (clone76)

   Plasmid

   #188579

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV-PGK-Cre

   Plasmid

   #172433

   Purpose

   Lentivirus, expresses Cre recombinase

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   Cre
   

   Use

   Lentiviral

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cbh_v5 AAV-CBE C-terminal

   Plasmid

   #137176

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Promoter

   Cbh

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cbh_v5 AAV-CBE N-terminal

   Plasmid

   #137175

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE N-terminal; U6-protospacer
   

   Use

   AAV

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cbh_v5 AAV-ABE C-terminal

   Plasmid

   #137178

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. WT1 KI gRNA1

   Plasmid

   #92312

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDlg/pRRE-int-MS2M

   Plasmid

   #166032

   Purpose

   Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   Gag-Pol-MS2
   

   Use

   Lentiviral

   Mutation

   D64V mutation within the integrase within Pol

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. H11-mScarletSIIN

   Plasmid

   #172438

   Purpose

   Hipp11 targeting vector, expresses mScarlet-SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   mScarlet-SIIN
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189924

    Purpose

    AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Mutation

    SaCas9 D10A, TadA ABE8e V106W

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cbh_v5 AAV-saCBE N-terminal

    Plasmid

    #137182

    Purpose

    AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE N-terminal
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cbh_v5 AAV-saCBE_KKH C-terminal

    Plasmid

    #137184

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE_KKH C-terminal
    

    Use

    AAV

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    Cbh

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cbh_v5 AAV-saCBE C-terminal

    Plasmid

    #137183

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE C-terminal
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WT1 KI gRNA2

    Plasmid

    #92313

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. H11-mScarletALG8

    Plasmid

    #172440

    Purpose

    Hipp11 targeting vector, expresses mScarlet-mALG8

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    mScarlet-mALG8
    

    Use

    Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-crRNA(Non-targeting)

    Plasmid

    #109324

    Purpose

    AAV vector expressing non-targeting AsCpf1 crRNA

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge