We narrowed to 1,221 results for: POLI

1. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

   Plasmid

   #184549

   Purpose

   Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ spike_del19

   Plasmid

   #155297

   Purpose

   Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

   Depositor

   Raffaele De Francesco

   Article

   Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

   Insert

   SARS-Cov2 spike_deleted (S SARS-Cov2)
   

   Use

   Generation of sars-cov-2-pseudotyped lentiviral p…

   Mutation

   Deletion in C-term 19 aa of spike

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mCherry-PARP1

   Plasmid

   #215136

   Purpose

   Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-PARP1-delM43;F44I

   Plasmid

   #211579

   Purpose

   CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains deletion of M43 and F44I

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-blast shGFP

   Plasmid

   #110419

   Purpose

   shGFP control, silence GFP gene, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA PolIII)

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. tet-pLKO.puro_shGFP

   Plasmid

   #110421

   Purpose

   shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet-pLKO.puro_shLuc

   Plasmid

   #110422

   Purpose

   shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer

    Plasmid

    #58837

    Purpose

    Expresses GFP-Tagged Human PLC delta PH domain in yeast

    Depositor

    Tim Levine

    Insert

    2x Phospolipase C delta1 PH domain (Plcd1 Rat)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSAV108

    Plasmid

    #226323

    Purpose

    Empty backbone plasmid for genomic insertions into H. neapolitanus neutral site

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-blast shLuc

    Plasmid

    #110409

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA PolIII)

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. tet-pLKO.puro_shHuR CDS

    Plasmid

    #110411

    Purpose

    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 (HuR)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-pLKO.puro_shGAC

    Plasmid

    #110423

    Purpose

    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. tet-pLKO.puro_shHuR 3UTR

    Plasmid

    #110412

    Purpose

    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 (HuR)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.puro_shHuR 3UTR

    Plasmid

    #110414

    Purpose

    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 (HuR)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-Nb11-P2

    Plasmid

    #236499

    Purpose

    Expresses anti-Apolipoprotein A1 nanobody Nb11 as a fusion protein with coiled coil P2 in bacteria.

    Depositor

    Alexander Green

    Article

    Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

    Insert

    Nb11-P2
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLiLucB

    Plasmid

    #31596

    Depositor

    Michele Calos

    Article

    Woodard et al PLoS One. 2010 . 5(6):e11367.

    Insert

    alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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