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We narrowed to 4,075 results for: PRS

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pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N

Plasmid

#147055

Purpose

Bacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-DmDCP1_1-127 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N

Plasmid

#147056

Purpose

Bacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G

Plasmid

#146403

Purpose

Bacterial Expression of DmDCP1_328-366-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut1 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G

Plasmid

#146404

Purpose

Bacterial Expression of DmDCP1_328-366-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut2 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G

Plasmid

#146405

Purpose

Bacterial Expression of DmDCP1_328-366-mut3

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut3 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G

Plasmid

#146406

Purpose

Bacterial Expression of DmDCP1_328-366

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK_E

Plasmid

#146214

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-G96C/A97C/U100C/U101C

Plasmid

#82328

Purpose

U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C

Plasmid

#81238

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C)

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45 (delta 154–164)

Plasmid

#126879

Purpose

Expresses crystallisation construct of human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

Human Cdc45 (CDC45 Human)

Use

Tags

No tag

Expression

Bacterial

Mutation

deletion amino acid 154-164

Promoter

T7 promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)

Plasmid

#106461

Purpose

Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

Plasmid

#106470

Purpose

Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

Plasmid

#68276

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4E) mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

RPS2

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_63/pRSETB

Plasmid

#51980

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only