Addgene

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CMV-mCh-kCAAX

Plasmid

#112267

Purpose

ITRACK Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

mCherry with KRas membrane targeting domain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Use

Tags

6xHis and CAAX

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Use

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-Lck-GCaMP2

Plasmid

#24794

Depositor

Baljit Khakh

Article

Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

Insert

Lck-GCaMP2

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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Myc-CD4-mCherry-HA-FRB

Plasmid

#100740

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD4

Use

Tags

FRB, HA, Myc, and mCherry

Expression

Mammalian

Mutation

Engineered to remove large part of extracellular …

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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PM-APEX2

Plasmid

#215557

Purpose

Stable, inducible expression in plasma membrane

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Lyn11-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-AktAR2

Plasmid

#184050

Purpose

Plasma membrane-targeted ExRai-AktAR2

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

PM-ExRai-AktAR2

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Budding Yeast, Rat)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-Sec61B

Plasmid

#141152

Purpose

Fluorescent labeling of ER membrane in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Sec61 beta (SEC61B Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

L79M on Sec61B

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2-iLID

Plasmid

#176125

Purpose

Plasma membrane localized iLID

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AktAR2

Plasmid

#184052

Purpose

Lysosome-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-ExRai-AktAR2

Use

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

Plasmid

#190236

Purpose

AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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B-MFF

Plasmid

#141158

Purpose

Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Use

Tags

GB

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GA-MFF

Plasmid

#141160

Purpose

Dimerization-dependent green fluorescent protein targeted to mitochondrial outer membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Use

Tags

GA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Use

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Lck-mCherry-RGS

Plasmid

#112935

Purpose

Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

Depositor

Dorus Gadella

Article

Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

Insert

p115RhoGEF-RGS domain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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