We narrowed to 1,247 results for: membrane gfp

1. pcDNA3-Lyn-dPlcR

   Plasmid

   #181832

   Purpose

   Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR
   

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-Neuromodulin-IRES-Puromycin

   Plasmid

   #134666

   Purpose

   Expresses EGFP-tagged plasma membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Neuromodulin (GAP43 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

   Plasmid

   #134866

   Purpose

   Expresses EGFP-tagged mutant of VAMP1

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Vesicle-associated membrane protein 1 (VAMP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 97-118

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lyn-tailed mCherry-SEpHluorin

   Plasmid

   #32002

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   Membrane-targeting sequence of Lyn kinase
   

   Expression

   Mammalian

   Available Since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS277

   Plasmid

   #34973

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Mutation

   V529N

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lyn-tdnano-HA

   Plasmid

   #136512

   Purpose

   Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   Lyn nano nano 3HA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SH4-FRB-mRFP

   Plasmid

   #100741

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   Fyn
   

   Tags

   FRB and mRFP

   Expression

   Mammalian

   Mutation

   SH4 domain only

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-mitoT

    Plasmid

    #174519

    Purpose

    Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

    Depositor

    Oleh Khalimonchuk

    Article

    Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

    Insert

    MitoT synthetic tether
    

    Tags

    6xHis and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDS240

    Plasmid

    #34966

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Mutation

    K533∆, high-affinity epitope

    Promoter

    GAL1

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mCh-kCAAX

    Plasmid

    #112267

    Purpose

    ITRACK Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    mCherry with KRas membrane targeting domain
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lck-mTurquoise2

    Plasmid

    #98822

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1-Lck-GCaMP2

    Plasmid

    #24794

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

    Insert

    Lck-GCaMP2
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myc-CD4-mCherry-HA-FRB

    Plasmid

    #100740

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD4
    

    Tags

    FRB, HA, Myc, and mCherry

    Expression

    Mammalian

    Mutation

    Engineered to remove large part of extracellular …

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PM-APEX2

    Plasmid

    #215557

    Purpose

    Stable, inducible expression in plasma membrane

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Lyn11-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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