We narrowed to 13,514 results for: Mpl

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pLenti-dSaCas9-KRAB-blast

Plasmid

#191654

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

dSaCas9-KRAB

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2-E148Q_U

Plasmid

#147643

Purpose

Mammalian Expression of HsDCP2-E148Q

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsDCP2-E148Q (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
GST-Rab8a-wt

Plasmid

#86078

Purpose

A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHis-parallel1-NLSH2BCas9

Plasmid

#112065

Purpose

Expression and purification of NLSH2BCas9 for fungal gene editing

Depositor

Jeff Coleman

Article

Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

Insert

NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger

Use

CRISPR

Tags

His tag

Expression

Bacterial

Mutation

Cas9 was codon optimized for Aspergillus niger

Promoter

promoter in vector

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4 (Retro)

Plasmid

#222690

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only