We narrowed to 57,330 results for: plasmid
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Plasmid#135974PurposeExpresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneExpressionMammalianAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pHRW32
Plasmid#130972PurposeHyPr plasmid with ble markerDepositorInsertempty backbone
ExpressionYeastAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)
Plasmid#139991PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationVRER=D1135V/G1218R/R1335E/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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p3E-Rac1-DN
Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact
Plasmid#109545PurposeMultisite gateway vector for 5' tagging with the actin binding motif LifeactDepositorInsertactin binding motif Lifeact
UseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJM019
Plasmid#134158PurposeRetroviral plasmid expressing GFP PSMA3DepositorAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8788
Plasmid#126922PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1DepositorInsertXdCrtI and HMG1
ExpressionYeastAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CB
Plasmid#12527DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele.Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1
Plasmid#134770PurposeA binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorPublicationTypeEmpty backboneExpressionPlantAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneExpressionPlantAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)1
Plasmid#127094PurposeBacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertmCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagsmCherryExpressionBacterialPromoterunknownAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q23-Myc-B
Plasmid#110488PurposeLentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationshort 3'UTR mutant huntingtin exon1 fragment…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
Plasmid#121831PurposepMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/LSD1 (open)
UseGateway Cloning and Synthetic BiologyTagsNLSAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-B2
Plasmid#122580PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C3
Plasmid#125061PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN6
UseSynthetic BiologyExpressionYeastAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C2
Plasmid#125060PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN5
UseSynthetic BiologyExpressionYeastAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only