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TAL2038

Plasmid

#39396

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(WT)

Plasmid

#1156

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Tags

GAL1:10 promoter

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG roGFP2-mito

Plasmid

#168509

Purpose

Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-roGFP2

Tags

roGFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4A-pCMV7.1

Plasmid

#155022

Purpose

N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4A (CUL4A Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1 S383A

Plasmid

#27160

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

TAD mutant: S383A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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hOtub1

Plasmid

#26959

Depositor

Cynthia Wolberger

Article

Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

Insert

Otub1 (OTUB1 Human)

Tags

His6 (TEV cleavable)

Expression

Bacterial

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Oct4i

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Uch37C88A

Plasmid

#19416

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

2XHA

Expression

Mammalian

Mutation

C88A

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Wnt3a (CT#169)

Plasmid

#13976

Depositor

Cliff Tabin

Article

Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

Insert

Wnt3a (Wnt3a Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRGEB32-BAR

Plasmid

#126072

Purpose

For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

Depositor

Charles Hunter

Article

Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-PIS

Plasmid

#119078

Purpose

Visualize localization of ER enzyme Phosphatidylinositol Synthase (PIS)

Depositor

Gia Voeltz

Article

English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

Insert

PIS (CDIPT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#118296

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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