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pDONR223_CTCF_p.N568K

Plasmid

#81612

Purpose

Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTCF (CTCF Human)

Use

Gateway entry vector

Mutation

N568K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_p.S214F

Plasmid

#82901

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ARAF (ARAF Human)

Use

Gateway entry vector

Mutation

S214F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82096

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARAF (ARAF Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_A4GALT_WT

Plasmid

#81835

Purpose

Gateway Donor vector containing A4GALT , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

A4GALT (A4GALT Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ING1_WT

Plasmid

#82213

Purpose

Gateway Donor vector containing ING1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ING1 (ING1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ASCL4_WT

Plasmid

#81909

Purpose

Gateway Donor vector containing ASCL4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ASCL4 (ASCL4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_p.C29F

Plasmid

#81307

Purpose

Gateway Donor vector containing CDKN1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway entry vector

Mutation

C29F, V109G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_p.R60del

Plasmid

#82740

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Mutation

R60del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_p.R35L

Plasmid

#82732

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Mutation

R35L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.E4K

Plasmid

#82860

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

E4K; E849D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

Y580F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_31

Plasmid

#60310

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_21

Plasmid

#60296

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CDKN2BAS enhancer (CDKN2B-AS1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_19

Plasmid

#60302

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

THADA enhancer (THADA Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE

Plasmid

#224936

Purpose

pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLCKO-CMV-TMPRSS2-IRES-Hygromycin

Plasmid

#237477

Purpose

Encodes full length human TMPRSS2 protein, along with hygromycin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

IRES-Hygromycin

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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