We narrowed to 23,290 results for: crispr

1. pCD017

   Plasmid

   #177322

   Purpose

   dCas9 expression

   Depositor

   James Carothers

   Article

   Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

   Insert

   dCas9
   

   Use

   CRISPR

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH113

   Plasmid

   #128210

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEC581

   Plasmid

   #174369

   Purpose

   RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   RFP with PAM rich sequence upstream promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23117

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_275

   Plasmid

   #179091

   Purpose

   nuclease Cas expression

   Depositor

   David Root, John Doench

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   Cas9-NG
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORE303N_CEN12RRFuzzy3MYB

   Plasmid

   #194441

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, RR, Fuzzy3MYB
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   MtU6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-BE4Gam

   Plasmid

   #138269

   Purpose

   Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BE4Gam
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Ef1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Blat-BSD

   Plasmid

   #163791

   Purpose

   The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKE014

   Plasmid

   #207908

   Purpose

   GG entry for zCas9i expression

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   SpCas9_introns_2xNLS-Pea3A(T) term
   

   Use

   CRISPR

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH49

   Plasmid

   #128211

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Rice codon optimized

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. A3D-Cas9n-UGI

    Plasmid

    #119137

    Purpose

    Base editor made from APOBEC3D

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3D-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT15001-AncBE4max

    Plasmid

    #163016

    Purpose

    plasmid expressing AncBE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPO635

    Plasmid

    #195297

    Purpose

    pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABC operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRRNA-G20-R20

    Plasmid

    #101822

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PABE-1

    Plasmid

    #115622

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    NLS-wtTadA-TadA7.10-nCas9-NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT15483_e-ABE8e

    Plasmid

    #174121

    Purpose

    Expresses ABE8e fused with eCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. A3F-Cas9n-UGI

    Plasmid

    #119138

    Purpose

    Base editor made from APOBEC3F

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3F-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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