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NODAL-T7TS-C312S

Plasmid

#115263

Purpose

For in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Mutation

C312S

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS-N72A-N199A

Plasmid

#115264

Purpose

For in vitro transcription of human NODAL open reading frame (with two mutated N-glycosylation sites) RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Mutation

N72A N199A

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Tags

6His

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-MYC-DYK

Plasmid

#115245

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Tags

MYC-DYK

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-matMYC

Plasmid

#115249

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E34: attB4-mCMVp>-attB1r

Plasmid

#162926

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

minimal CMV IE1 promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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