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We narrowed to 63,769 results for: cloning

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Showing: 11701 - 11720 of 63769 results

  11. Mucolipin1 D471-472K-pHcRed C1

    Plasmid
    #62961
    Purpose
    full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1
    Depositor
    Paul Luzio
    Publication
    Pryor et al Traffic. 2006 Oct;7(10):1388-98.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mucolipin-1 (MCOLN1 Human)
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    D471/472K (and two silent mutations T77 ACA inst…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. pHAGE-CMV-Flag-AAK1_IDG-K

    Plasmid
    #135280
    Purpose
    Gateway destination clone of AAK1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-AAK1 (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135280-plasmid-map-sequence-id-311570

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  13. p663-UBC-V5-TurboID-V5-STOP_IDG-K

    Plasmid
    #158159
    Purpose
    Gateway destination clone of TurboID-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158159-plasmid-map-sequence-id-309970

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  14. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid
    #135261
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135261-plasmid-map-sequence-id-268068

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  15. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid
    #135263
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135263-plasmid-map-sequence-id-268067

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  17. pME-p3E-HyPer7

    Plasmid
    #194289
    Purpose
    Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV
    Depositor
    Rob Parton
    Publication
    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyper7
    Use
    Gateway Cloning
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    194289-plasmid-map-sequence-id-380038

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  19. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  20. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173202-plasmid-map-sequence-id-339519

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Showing: 11701 - 11720 of 63769 results