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EGFP-UVRAG S498D

Plasmid

#86742

Purpose

N-terminal EGFP-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

EGFP

Expression

Mammalian

Mutation

S498D

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pNB785

Plasmid

#60164

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRKVSV Rac T17N

Plasmid

#50537

Depositor

Kenneth Yamada

Article

Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

Insert

small GTPase, mutated (RAC1 Human)

Tags

2X VSV

Expression

Mammalian

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pNB787

Plasmid

#60152

Purpose

Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB789

Plasmid

#60166

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR FOXP3 WWRR

Plasmid

#16364

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Expression

Bacterial

Mutation

WWRR: T359W, N361W, E399R, E401R

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL VV458/460PP

Plasmid

#61368

Purpose

Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with VV458/460P mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P and V460to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-hRIP1-FL I539P

Plasmid

#61380

Purpose

Expresses Human full length RIPK1with I539P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I539P mutation (RIPK1 Human)

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I539 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
mlp-hCHES1sh

Plasmid

#51845

Purpose

shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

shRNA against CHES1 (FOXN3 Human)

Use

Retroviral

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pWZL-rCHES1

Plasmid

#51847

Purpose

encodes a CHES1 mRNA that is resistant to shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-S35

Plasmid

#39511

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-G37

Plasmid

#39512

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and E37G

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-C40

Plasmid

#39515

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
EYFP-Abr NE/AA

Plasmid

#36419

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EYFP

Expression

Mammalian

Mutation

N245A, E246A

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
delta-LIM Isl1

Plasmid

#23261

Depositor

Samuel Pfaff

Article

Thaler et al Cell. 2002 Jul 26. 110(2):237-49.

Insert

Isl1 (Isl1 Rat)

Use

Avian/xenopus/zebrafish

Tags

Lamda-dimer domain

Expression

Mammalian

Mutation

Delta LIM

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pENTR FOXP3 WRR

Plasmid

#16365

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Expression

Bacterial

Mutation

WRR: N361W, E399R, E401R

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pENTR FOXP3 EARR

Plasmid

#16367

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Expression

Bacterial

Mutation

EARR: N361E, H365A, E399R, E401R

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.(mem).iGlucoSnFR2

Plasmid

#244096

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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