We narrowed to 18,078 results for: comp

1. YFP NLS Beta-Actin G13R

   Plasmid

   #60615

   Purpose

   Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   G13R

   Promoter

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Brg1 Del2 pET28a

   Plasmid

   #122116

   Purpose

   Expression Brg1 amino acids 1-837 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   6XHis and His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Brg1 Del3 pET28a

   Plasmid

   #122117

   Purpose

   Expression Brg1 amino acids 836-1446 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N3/hArf6(WT)-EGFP

   Plasmid

   #79423

   Purpose

   Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. INCTbiosyn-pEF-GFP(rc)9

   Plasmid

   #127508

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. INCTbiosyn-pEF-GFP(rc)13

    Plasmid

    #127509

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. INCTbiosyn-pEF-GFP(rc)2

    Plasmid

    #127504

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE6a

    Plasmid

    #207851

    Purpose

    Mammalian expression of PE6a prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6a
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

    Plasmid

    #185446

    Purpose

    Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG_Xph20-eGFP-CCR5TC

    Plasmid

    #135530

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YFP-Parkin-IRES-zeo

    Plasmid

    #61728

    Purpose

    Expression of human Parkin fused to eYFP

    Depositor

    Stephen Tait, Douglas Green

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_Xph20-SNAPf-CCR5TC

    Plasmid

    #135537

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OPEN Beta2-microglobulin

    Plasmid

    #215657

    Purpose

    E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN Beta2-microglobulin (B2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 31 to Cysteine

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG_Xph18-eGFP-CCR5TC

    Plasmid

    #135529

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge