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pEGFP-C1 hSlp4-5-4a

Plasmid

#40045

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-5-4a (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Slp4 linker substituted by Slp5 linker domain (s…

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a W118S

Plasmid

#40042

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Tryptophane 118 to Serine

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a V21A

Plasmid

#40041

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Valine 21 to Alanine

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a SHD

Plasmid

#40034

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a SHD (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

SHD domain (1-143)

Promoter

CMV promoter

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMEF2c-AHF-Dre-nls (JDW 35)

Plasmid

#242585

Purpose

The Mef2c anterior heart field promoter driving expression of Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE-nls

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ULK1-ADA

Plasmid

#249534

Purpose

Expressing human full length ULK1 with P433A/V434D/P435A mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

Flag-tag and strep-tag

Expression

Mammalian

Mutation

P433A, V434D and P435A

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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huCofilin K22Q SSR-mRFP

Plasmid

#252617

Purpose

Expresses shRNA resistant human cofilin with K22Q mutation that reduces nuclear uptake and serves as a cofilactin rod reporter

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q; silent mutations at nt 67 to 75 in which TC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pEHE51

Plasmid

#250522

Purpose

Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.

Depositor

Ralph Isberg

Article

Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0

Insert

Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-synaptojanin1(D730A,498-901)-CAAX

Plasmid

#241304

Purpose

Mammalian expression of truncated form of 5-phosphatase-dead synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa, D730A

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Lyn-FRB_star-P2A-mVenus-FKBP-5ptase(D343A)

Plasmid

#241306

Purpose

Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase(phosphatase-dead mutant) of INPP5E

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

INPP5E (INPP5E Human)

Tags

mVenus, FKBP

Expression

Mammalian

Mutation

214-644 aa, D343A

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K264A,K265A,R210H,K84TAG)-mCherry

Plasmid

#241302

Purpose

Mammalian expression of syntaxin-1(K264A,K265A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K264A,K265A,K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLVXNeo_SPARC-mSc-SBP

Plasmid

#234885

Purpose

Expresses mScarlet-i and SBP tagged human SPARC for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Article

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

SPARC (SPARC Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-mGrm3-WPRE

Plasmid

#224236

Purpose

AAV expression of mGrm3 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mGrm3 (Grm3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-DreERT2 (JDW 196)

Plasmid

#242586

Purpose

The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-human Cyclin L1 alpha-IRES-EGFP

Plasmid

#240140

Purpose

Express Cyclin L1 alpha

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 alpha (CCNL1 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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