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pVRC8400-SARS-CoV-2-S2P-B.1.618-AVI

Plasmid

#176336

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.618 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Y145del, H146del, E484K, D614G

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-B.1.1.7-AVI

Plasmid

#176424

Purpose

Expresses SARS-CoV-2 NTD domain from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-B.1.1.7 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

69-70 del, 144del

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-B.1.351-AVI

Plasmid

#176426

Purpose

Expresses SARS-CoV-2 NTD domain from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-B.1.351 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L18F, D80A, D215G, 242-244del, R246I

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-T95I-D253G-AVI

Plasmid

#176432

Purpose

Expresses SARS-CoV-2 NTD domain containing T95I-D253G mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-T95I-D253G (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T95I, D253G

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-B.1.429-AVI

Plasmid

#176328

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.429 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.429 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

W152C, L452R, D614G

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI

Plasmid

#176329

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.429 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T95I, D253G, S477N, D614G, A701V

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI

Plasmid

#176330

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.429 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T95I, D253G, E484K, D614G, A701V

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI

Plasmid

#176333

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.617.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE1

Plasmid

#101439

Purpose

Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE1 (FOXE1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Promoter

kdrl

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway cloning

Tags

mTagBFP2

Mutation

mutant KRAS4A-G12D

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_WT

Plasmid

#81898

Purpose

Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR141_WT

Plasmid

#81876

Purpose

Gateway Donor vector containing GPR141 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPR141 (GPR141 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CNBD1_WT

Plasmid

#81877

Purpose

Gateway Donor vector containing CNBD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CNBD1 (CNBD1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HERPUD2_WT

Plasmid

#81880

Purpose

Gateway Donor vector containing HERPUD2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HERPUD2 (HERPUD2 Human)

Use

Gateway entry vector

Mutation

A108T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKZF5_WT

Plasmid

#81855

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Mutation

M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C20orf72_WT

Plasmid

#81806

Purpose

Gateway Donor vector containing C20ORF72 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C20orf72 (MGME1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GZMA_WT

Plasmid

#81792

Purpose

Gateway Donor vector containing GZMA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GZMA (GZMA Human)

Use

Gateway entry vector

Mutation

Y5N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRH4_WT

Plasmid

#81786

Purpose

Gateway Donor vector containing HRH4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRH4 (HRH4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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