We narrowed to 5,164 results for: pcDNA3.1+

1. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

   Plasmid

   #171436

   Purpose

   Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-Lifeact
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

   Plasmid

   #171435

   Purpose

   Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   Lifeact-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

   Plasmid

   #194680

   Purpose

   CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Hpep1-betaArrestin2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 His-NLRP1 codon-optimized

   Plasmid

   #166833

   Purpose

   Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Hygro EYFP H148Q/I152L

   Plasmid

   #25874

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1-HsPcdh21-Myc-Flag-His

   Plasmid

   #210818

   Purpose

   Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   Myc, 3x Flag, 10x His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-BCR-ABL-p210-FLAG

   Plasmid

   #205619

   Purpose

   Expression of BCR-ABL oncogene in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

   Insert

   BCR-ABL oncogene, p210 isoform b3a2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225240

    Purpose

    Mammalian expression of iPASnFR-L in mitochondria

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-BCR-ABL-p190-FLAG

    Plasmid

    #205620

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p190 isoform b3a2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T117M- Please see depositor comments

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. XE248 pCDNA3.1+zeo-VSV-KLHL12

    Plasmid

    #16761

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

    Plasmid

    #183249

    Purpose

    Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    Anti-Her2 scFvFc (FRP5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225246

    Purpose

    Mammalian expression of iPASnFR-L in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1V5-His Snk/Plk2 T239A

    Plasmid

    #22219

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. (133) pcDNA3.1-Nup62-Flag-EPEA

    Plasmid

    #160744

    Purpose

    Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    Nup62-Flag-EPEA
    

    Use

    Tags

    EPEA and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

    Plasmid

    #73207

    Purpose

    Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    KozATG-dL5-2XG4S-mCer3
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

    Plasmid

    #225244

    Purpose

    Mammalian expression of iPASnFR-L fused to Rab5

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225239

    Purpose

    Mammalian expression of iPASnFR-H in mitochondria

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

21. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75203

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1-Flag-H2A K118-119R

    Plasmid

    #63564

    Purpose

    Expression of human H2A with K118R and K119R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 118 and Lysine 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

    Plasmid

    #171437

    Purpose

    Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    c-Fos-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 FLAG-SIV-MAC251-vpx

    Plasmid

    #115842

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Use

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

    Plasmid

    #225587

    Purpose

    Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-vhhGFP4-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3.1-Flag-H2A K13-15R

    Plasmid

    #63563

    Purpose

    Expression of human H2A with K13R and K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 13 and Lysine 15 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

    Plasmid

    #171428

    Purpose

    Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

    Plasmid

    #171438

    Purpose

    Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-c-Jun
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge