2,226 results

1. FUGW-RUSH-LAMP1-V5-APEX2

   Plasmid

   #228524

   Purpose

   Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

   Depositor

   Ginny Farias

   Article

   Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP), Streptavidin-…

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-RUSH-LAMP1-mScarleti

   Plasmid

   #228526

   Purpose

   Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

   Depositor

   Ginny Farias

   Article

   Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP), Streptavidin-…

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-mGold2s-Lysosome-N-20

   Plasmid

   #231770

   Purpose

   Visualization of lysosome in mammalian cells using mGold2s

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2s-Lysosome-N-20 (Lamp1 Rat)
   

   Use

   Tags

   mGold2s

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-mGold2t-Lysosome-N-20

   Plasmid

   #231778

   Purpose

   Visualization of lysosome in mammalian cells using mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t-Lysosome-N-20 (Lamp1 Rat)
   

   Use

   Tags

   mGold2s

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Kif1A-EGFP-SNAP

   Plasmid

   #229851

   Purpose

   Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif1A-EGFP-SNAP (Kif1a Rat)
   

   Use

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-APOBEC1-EGFP

   Plasmid

   #209324

   Purpose

   AAV packaging vector containing a APOBEC1 expression, a P2A-EGFP expression cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMVtrunc F-tractinN9-39-msfGFPΔN7

   Plasmid

   #231552

   Purpose

   Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   F-tractin (Itpka Rat)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdnCas3-APOBEC1

   Plasmid

   #229537

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   dnCas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas3-APOBEC1

   Plasmid

   #229536

   Purpose

   pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   Cas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MT2

    Plasmid

    #223330

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
    

    Use

    Tags

    3HA-TurboID-EGFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MT3

    Plasmid

    #223331

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
    

    Use

    Tags

    3HA-EGFP-miniTurboID

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MT4

    Plasmid

    #223332

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
    

    Use

    Tags

    3HA-miniTurboID-EGFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IPU-SNAP-Omp25

    Plasmid

    #228137

    Purpose

    Expresses SNAP-Omp25 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    transmembrane region (C-terminus)

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

    Plasmid

    #199580

    Purpose

    Expresses GCaMP8f in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    His-DIO GCaMP8f
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. macroH2A1.1-GFP (pc2188)

    Plasmid

    #223707

    Purpose

    Expression of macroH2A1.1 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.1-GFP (Macroh2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. macroH2A1.2-GFP (pc2189)

    Plasmid

    #223708

    Purpose

    Expression of macroH2A1.2 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.2-GFP (Macroh2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. macroH2A1.1-F192V-GFP (pc5114)

    Plasmid

    #223437

    Purpose

    Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.1-F192V (Macroh2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. macroH2A1.2-F192V-GFP (pc5115)

    Plasmid

    #223438

    Purpose

    Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.2-F192V (Macroh2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of phenylalanine 192 to valine (F192V)

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIG2-IRES-Lamp1-mNeon Green

    Plasmid

    #219441

    Purpose

    Expresses Lamp1 fused to mNeon Green

    Depositor

    Erika Holzbaur

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MUNC13-1 MUN

    Plasmid

    #218889

    Purpose

    Expresses the MUN domain from MUNC13-1 in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    MUNC13-1 MUN domain (Unc13a Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. H6Gb1-CREB(1-280) C90A, T172C

    Plasmid

    #217983

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    C90A, T172C

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. icLCT

    Plasmid

    #223179

    Purpose

    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)

    Depositor

    Andreas Stuermer

    Insert

    cytosolic intestinal lactase protein fragment (Lct Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

    Plasmid

    #217329

    Purpose

    Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S729A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid

    #217328

    Purpose

    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid

    #217327

    Purpose

    Purification of catalytically-dead rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    S729A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac HTB His-TEV-rDPP9

    Plasmid

    #217326

    Purpose

    Purification of rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge