We narrowed to 31,888 results for: Eng

1. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

   Plasmid

   #126739

   Purpose

   yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2, acs1*, ald6
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, S629N (please see depositor comm…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-crRNA(TBK1)

   Plasmid

   #109322

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TBK1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

   Plasmid

   #195046

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. H11-mScarletLAMA4

   Plasmid

   #172439

   Purpose

   Hipp11 targeting vector, expresses mScarlet-mLAMA4

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   mScarlet-mLAMA4
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Coexpressed Free ClLIS1 (M)

   Plasmid

   #182484

   Purpose

   Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS(M)
   LS
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Coexpressed Free deadFPPS-NES

   Plasmid

   #182492

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   deadFPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-eGFP-uTEV3

   Plasmid

   #234320

   Purpose

   Mammalian expression of uTEV3 tagged with an eGFP marker

   Depositor

   Mateo Sánchez

   Article

   Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

   Insert

   eGFP-uTEV3
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Mutations respective to the wild type TEV: S219V …

   Promoter

   CMV

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172434

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    Lentiviral

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-sgfiller-eCas9-T2A-BlastR

    Plasmid

    #172437

    Purpose

    Expresses eCas9, BlastR and sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    eCas9
    BlastR
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid

    #185916

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ad:2CMV_A2R2-N25K3

    Plasmid

    #122242

    Purpose

    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid

    #185915

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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