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155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Mutation

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G228A

Plasmid

#84926

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G228A

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G250A

Plasmid

#84925

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G250A

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTH734-2µ-RLuc/stopCFLuc

Plasmid

#40609

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase (nonsense mutant)
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHE Short

Plasmid

#137697

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHG Short

Plasmid

#137699

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTH752-CEN-RLuc/min103maxCFLuc

Plasmid

#38218

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 103 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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hTph2p-Luc

Plasmid

#223526

Purpose

Firefly-luciferase reporter expression driven by human Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Tph2 promoter (TPH2 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

hTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-Luc

Plasmid

#223663

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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