We narrowed to 1,558 results for: pBI

1. pAcGFP1-N1-RvCAHS3-GS

   Plasmid

   #192460

   Purpose

   Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Bsn-GFP KI

   Plasmid

   #139665

   Purpose

   Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRT498-OtUBD(V203D)

   Plasmid

   #190090

   Purpose

   Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD(V203D)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP1-N1-RvCAHS12-GS

   Plasmid

   #192462

   Purpose

   Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Tagger

   Plasmid

   #129396

   Purpose

   Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

   Insert

   rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE GFP-Bsn KI

   Plasmid

   #139664

   Purpose

   Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORANGE GFP-Pclo KI

   Plasmid

   #139657

   Purpose

   Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniSOG-H2B-C-10

   Plasmid

   #57769

   Purpose

   Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mVenusC-Cavin3

   Plasmid

   #108890

   Purpose

   Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   Cavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
   

   Use

   Tags

   mVenus156-239

   Expression

   Mammalian

   Mutation

   altered codon usage

   Promoter

   Available since

   March 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE Rims1-GFP KI

    Plasmid

    #131494

    Purpose

    Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRB-mCherry-EEA1

    Plasmid

    #222419

    Purpose

    To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

    Plasmid

    #182252

    Purpose

    Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PIK3R1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mVenusN-Cavin3

    Plasmid

    #108889

    Purpose

    Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
    

    Use

    Tags

    mVenus1-155

    Expression

    Mammalian

    Mutation

    altered codon usage

    Promoter

    Available since

    March 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PARD3_PDZ_3

    Plasmid

    #103959

    Purpose

    Protein expression and purification of PAR3 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PARD3 (PARD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpeCas9-HF

    Plasmid

    #192141

    Purpose

    Expresses highly specific SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9-HF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    SpeCas9-HF (R247A, N415A, S421A)

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PTPN13_PDZ_4

    Plasmid

    #103922

    Purpose

    Protein expression and purification of PTN13 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPyCAG-NE-mKate2-IRES-Hph

    Plasmid

    #183616

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

    Insert

    mKate2
    

    Use

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-mCherry-miniSOG-Zyxin-6

    Plasmid

    #57802

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge