-
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pET28-sfGFP-DogTag Loop B
Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13, MG-26-82)
Plasmid#110746PurposeBacterial expression of human USP30 (construct 13)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350S, I353E + insertion deletion: 64-178;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop A
Plasmid#171776PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-SpyTag003 Loop A
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues V…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13i, C77A, MG-31-28)
Plasmid#110747PurposeBacterial expression of human USP30 (construct 13i)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationC77A, F348D, M350S, I353E + insertion deletion: 6…PromoterT7Available sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NEMOL329P
Plasmid#11969DepositorInsertNEMOL329P (IKBKG Human)
UseTagsHisExpressionBacterialMutationLeucine329 to ProlinePromoterAvailable sinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop C
Plasmid#171781PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-DogTag Loop C
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only