Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,485 results for: pcas9

Showing: 1161 - 1180 of 2485 results

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pEPOR2KN0016

Plasmid

#117588

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0015

Plasmid

#117587

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0014

Plasmid

#117586

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0013

Plasmid

#117585

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0012

Plasmid

#117584

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0011

Plasmid

#117583

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0010

Plasmid

#117582

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0009

Plasmid

#117581

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0008

Plasmid

#117580

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0004

Plasmid

#117579

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0003

Plasmid

#117578

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0001

Plasmid

#117576

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0015

Plasmid

#117525

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (no stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Mutation

D1135E

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-BsaIx2-DD-Cas9

Plasmid

#75380

Purpose

Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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p101_CRISPRai_PiggyBac

Plasmid

#213777

Purpose

Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

VPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator

Use

CRISPR

Promoter

EF1a (EF-1-alpha intron a) and Tet_On_TRE

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Showing: 1161 - 1180 of 2485 results

Results per page:

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