We narrowed to 19,638 results for: IRE
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Plasmid#155049PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
UseCRISPRAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
3389 pcDNA3 Bid p22
Plasmid#8807DepositorInsertBid p22 (Bid Mouse)
ExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CRY-tTA (B702)
Plasmid#92032PurposeExpresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activatorDepositorInsertCRY2 (CRY2 Mustard Weed)
TagstTA2 (TetR fused to VP64)ExpressionMammalianMutationNLS on CRY2 is mutatedPromoterCMVAvailable SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase S1226A/ C2029A
Plasmid#59823PurposeAllows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationS1226A/ C2029APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_J
Plasmid#146709PurposeInsect Expression of DmNot1DepositorInsertDmNot1 (Not1 Fly)
ExpressionInsectMutationThree non silent mutations N605S, K759M, G1999S a…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL-T500
Plasmid#92314PurposeExpression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LK4-Ets2
Plasmid#44606DepositorAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA_81-236-FLAG (pBS0297)
Plasmid#185328PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA_81-236-FLAG
TagsFLAGExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A3-VN
Plasmid#135506PurposeMammalian expression of VN-fused and myc-tagged HLA-A*03:01DepositorInsertHLA-A*03:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
(363) pcDNA3.1-Nup62-Ubc-Flag-EPEA
Plasmid#168098PurposeNucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tagDepositorInsertNup62-Ubc-Flag-EPEA
TagsEPEA-tag, Flag-tag, and Ubc-TagExpressionMammalianMutationP229A, S283T on Nup62 native sequencePromoterT7/cmvAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc
Plasmid#138367PurposeLentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD5-Strep-HA
Plasmid#113493PurposeExpression of human UBXD5 with C-terminal strep-HA tagDepositorInsertUBXD5 (UBXN11 Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L16A/L17A
Plasmid#13478DepositorInsertMKP-1L16A/L17A (Dusp1 Mouse)
TagsEGFPExpressionMammalianMutationChanged Leucine 16 to Alanine; changed Leucine 1…Available SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
3392 pcDNA3 Bid p15
Plasmid#8808DepositorInsertBid p15 (Bid Mouse)
ExpressionMammalianAvailable SinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-ΔCT
Plasmid#64062Purposemyc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationDeletion of C-terminal Asp338–Ser443Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Plxnb2-ISH-2kb
Plasmid#68036PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertPlexin-B2 (Plxnb2 Mouse)
UseMrna in situ hybridization probeAvailable SinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL 1-187-pRc/CMV
Plasmid#22079DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationdeletion mutant covering only aa 1-187 of wt VHL …Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only