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pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Publication

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

GFP

Expression

Mammalian

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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human p53-(355-393)

Plasmid

#24870

Depositor

Cheryl Arrowsmith

Publication

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 355-393.

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-CCL21-V5

Plasmid

#11609

Depositor

Steven Johnson

Insert

chemokine (C-C motif) ligand 21 (CCL21 Human)

Tags

V5

Expression

Mammalian

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF9b

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-fDIO-mCherryP2ACre

Plasmid

#161773

Purpose

Flp-Dependent mCherry and Cre expression

Depositor

Baoji Xu

Publication

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Insert

mCherry-P2A-Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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HP35 tension sensor

Plasmid

#101250

Purpose

Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

Depositor

Carsten Grashoff

Publication

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-mHRI-FLAG-IP

Plasmid

#101791

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse HRI

Depositor

Seiichi Oyadomari

Publication

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Hri (Eif2ak1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hGAPDH-rLuc

Plasmid

#82479

Purpose

reporter of GAPDH expression

Depositor

Albee Messing

Publication

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

GAPDH promoter (GAPDH Human)

Use

Luciferase

Expression

Bacterial

Promoter

hGAPDH

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CyTERM-mGFP

Plasmid

#62237

Purpose

ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

Depositor

Erik Snapp

Publication

Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

Insert

CyTERM-mGFP

Tags

mGFP

Expression

Mammalian

Mutation

signal anchor of rabbit cytochrome p450 and monom…

Promoter

CMV

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pQHA-USP11 CS puroR

Plasmid

#46750

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Publication

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt10A

Plasmid

#35920

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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YTIP-GFP

Plasmid

#42238

Depositor

William Pu

Publication

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YTIP-GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sh-RNA hAtg13

Plasmid

#31971

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SHMT2

Plasmid

#25479

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SHMT2 (SHMT2 Human)

Tags

His

Expression

Bacterial

Mutation

A269T

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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GABARAPL1

Plasmid

#25215

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubh57.003.111 (GABARAPL1 Human)

Tags

His

Expression

Bacterial

Available since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMIR-9

Plasmid

#25037

Depositor

Bob Weinberg

Publication

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 binding site (MIR9-1 Human)

Use

Luciferase

Expression

Mammalian

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles