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Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(T31N)-HA

Plasmid

#79405

Purpose

Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-mCherry

Plasmid

#79406

Purpose

Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pSG5-hAR(16)

Plasmid

#89120

Purpose

mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out

Depositor

Elizabeth Wilson

Article

Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.

Insert

hAR (AR Human)

Expression

Mammalian

Mutation

internal EcoRI site removed

Promoter

SV40

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pPotef-cdc3CB16-NatR

Plasmid

#86465

Purpose

Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

Depositor

Michael Feldbrügge

Article

Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

Insert

Cdc3

Use

Ustilago maydis expression

Tags

mCherry

Expression

Bacterial

Promoter

Potef

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-CrxshRNA

Plasmid

#73987

Purpose

Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Crx gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA1

Plasmid

#73981

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pTXB1-FIS

Plasmid

#51564

Purpose

Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

Depositor

Sankar Adhya, Francisco Malagon

Insert

FIS

Tags

Intein-Chiting Binding Domanin

Expression

Bacterial

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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AKR7A2

Plasmid

#39130

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AKR7A2 (AKR7A2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1611

Plasmid

#29276

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple139 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Jan. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1612

Plasmid

#29277

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple140 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Jan. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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GST-RB (561-673)

Plasmid

#31495

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 561-673

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-shDusp5-2-H2Kb2

Plasmid

#17853

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

shDusp5 (DUSP5 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pOX + kqt2

Plasmid

#16137

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-2 Nematode)

Use

Xenopus oocyte expression

Mutation

Deletion of aa1-38 compared to GenBank NP_509392.3

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBpuro - HA KLF4 FL delta K5S

Plasmid

#34592

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

LTR

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-RNF152-IRES-mScarlet-I

Plasmid

#215373

Purpose

Bicistronic expression of EGFP-tagged lysosome membrane protein RNF152, along with mScarlet-I expressed as an expression control.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

RNF152 (RNF152 Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-FRCaMPi

Plasmid

#232838

Purpose

AAV transfer plasmid for Syn-mediated expression of FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaFRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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