We narrowed to 29,458 results for: REP

1. pJYDN2p

   Plasmid

   #162455

   Purpose

   Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-DnbALFA (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA nanobody and HA and myc

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKI1.1

   Plasmid

   #85807

   Purpose

   CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   May 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSK619

   Plasmid

   #205134

   Purpose

   Expression of SnxA-GFP in Dictyostelium

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Use

   Dictyostelium expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLV Gag-CFP

   Plasmid

   #1812

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   Gag (gag MuLV)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJWV102-wtcas9sp

   Plasmid

   #99612

   Purpose

   Plasmid containing wild-type cas9sp

   Depositor

   Morten Kjos, Jan-Willem Veening

   Article

   van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

   Insert

   cas9sp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PczcD

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC.Taq.1Amb

   Plasmid

   #102794

   Purpose

   Expresses Taq DNA polymerases with a premature stop codon

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Taq DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   W168Stop; *(see below)

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG506-mito-frFAST

   Plasmid

   #160057

   Purpose

   Expresses mito-frFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

   Insert

   Mito-frFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CS2 Flag-Smad3 (3A)

   Plasmid

   #14964

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

   Insert

   Smad3 (3A) (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   Three Ser to Ala mutations in the phosphorylation…

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD3-proD/mClover/nat

   Plasmid

   #120330

   Purpose

   Bacterial mClover labeling

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   mClover
   

   Use

   Tags

   D103N (see comment below)

   Expression

   Bacterial

   Mutation

   Promoter

   (None)

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL0_19 [panARS ORI]

    Plasmid

    #198932

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    panARS ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD227

    Plasmid

    #113318

    Purpose

    AraC-pBAD-dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    R93A

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX165-evoCas9

    Plasmid

    #140569

    Purpose

    Expresses evoCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    M495V Y515N K526E R661Q

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-tdTom-SP

    Plasmid

    #48677

    Purpose

    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Sp6

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-P-RAN-Gelsolin

    Plasmid

    #106413

    Purpose

    P-RAN-Gelsolin (RANbody Gelsolin::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-Gelsolin
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG499-FRB-N-frFAST

    Plasmid

    #160059

    Purpose

    Expresses FRB-N-frFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

    Insert

    FRB-N-frFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJS010

    Plasmid

    #188333

    Purpose

    PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mKate2::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJJS004

    Plasmid

    #188327

    Purpose

    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mCherry::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pG6(5'Pro) (P#1477)

    Plasmid

    #14659

    Depositor

    Matija Peterlin

    Article

    Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

    Insert

    UAS repeats
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. LRT2B-AdGO

    Plasmid

    #136910

    Purpose

    Lentivirus expressing AdGO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgAdGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-AkCas12b-2AeGFP

    Plasmid

    #121946

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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