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pv6_TAF3_2xPhd

Plasmid

#179399

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3_2xPhd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PTGS1-bio-His

Plasmid

#53414

Purpose

Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PTGS1 (PTGS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a(R887E)-DNMT3l

Plasmid

#154141

Purpose

Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Tags

HA and sfGFP

Expression

Mammalian

Mutation

R887E

Promoter

SFFV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 Cyclin E1

Plasmid

#109348

Purpose

virus packaged, doxycyline inducible expression of Cyclin E1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cyclin E1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-GRAM1b

Plasmid

#211702

Purpose

Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b (pLJM1-EGFP-GRAM1b)

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVFLAG-ZFP423

Plasmid

#24764

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

Plasmid

#154003

Purpose

Vector expressed CasRx and sgRNA_LACZ for AAV package

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

AAV and CRISPR

Promoter

EFS

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

SgRNA expression system

Use

CRISPR

Promoter

U6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 BLTP3A

Plasmid

#241247

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) as a C-terminal fusion to EGFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) (BLTP3A Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMax_mPlum_C207G/T208C

Plasmid

#177831

Purpose

Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum_C207G/T208C

Expression

Mammalian

Mutation

C207G + T208C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Pa01 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

3XFLAG-Cas9

Expression

Mammalian

Promoter

CBh; U6

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET29_LuxSit_i

Plasmid

#194299

Purpose

Bacterial expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Expression

Bacterial

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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