We narrowed to 168,368 results for: addgene

Showing: 11841 - 11860 of 168368 results

Results per page:

Sort results by:

sgRNA BRCA2 R2896C

Plasmid

#139511

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2896C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1 intron 1 gRNA s3

Plasmid

#132395

Purpose

Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
human ETV6 gRNA-3

Plasmid

#133403

Purpose

human ETV6 gRNA-3 is a 20-nt gRNA expression plasmid targeting the second ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-shCrh2

Plasmid

#132710

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(2) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-TIRAP-cGASΔN-HA

Plasmid

#130921

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

TIRAP-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the N ter…

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-Fyn-cGASΔN-HA

Plasmid

#130922

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Fyn-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the dual …

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
289aa ELL2

Plasmid

#127266

Purpose

For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu. Contains 289…

Promoter

T7

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Xba ELL2 Met133, 138, 186 to Ileu

Plasmid

#127269

Purpose

For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu HA tag after M186

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Sph ELL2 Met133, 138, 186 to Ileu

Plasmid

#127271

Purpose

For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu with HA tag after 8th Met

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh105

Plasmid

#106775

Purpose

expression of gRNA targeting B3GALT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT1 (B3GALT1 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-HA-Tbkbp1 1-280

Plasmid

#125178

Purpose

Retroviral expression of mouse Tbkbp1 (1-280)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-280

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-HA-Tbkbp1 1-330

Plasmid

#125179

Purpose

Retroviral expression of mouse Tbkbp1 (1-330)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-330

Promoter

CMV

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh2

Plasmid

#106686

Purpose

expression of gRNA targeting GALNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT2 (GALNT2 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh110

Plasmid

#106769

Purpose

expression of gRNA targeting B3GALTL (B3GLCT)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALTL (B3GLCT) (B3GLCT Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh106

Plasmid

#106776

Purpose

expression of gRNA targeting B3GALT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT2 (B3GALT2 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh109

Plasmid

#106768

Purpose

expression of gRNA targeting B3GALT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT6 (B3GALT6 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh146

Plasmid

#106805

Purpose

expression of gRNA targeting FKTN

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FKTN (FKTN Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge