167,150 results
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Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneTagsFlag-APEX2ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA
Plasmid#180590PurposeOpto-cytTrkBDepositorInsertTrkB
UseAAVMutationPHR(E281A)Available SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535
Plasmid#46886PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…ExpressionBacterialPromoterPnisAAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mCherry-T2A-Puro
Plasmid#72264PurposeExpress mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.DepositorInsertmCherry-T2A-Puro
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PAmCherry1-PH_PLCd1
Plasmid#92167PurposePI(4,5)P2 single molecule fluorescent reporterDepositorInsertiRFP-PAmCherry1-PH_PLCd1
TagsiRFP-PAmCherry1ExpressionMammalianPromoterCMVAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
B*57:03:01
Plasmid#203260PurposeOverexpress HLA alleleDepositorInsertB*57:03:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only