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pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

Plasmid

#122472

Purpose

Dox-inducible GFP (N-terminal tag) SCAF8 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 (SCAF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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actb2-lifeact-mKate2

Plasmid

#109750

Purpose

Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the actin binding lifeact sequence

Use

Zebrafish plasmids

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pInducer21-3xFlag-S208C-MEFV

Plasmid

#167019

Purpose

Lentiviral plasmid with 3xFlag-pyrin (S208C/PAAND variant) under the control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

Serine 208 changed to Cysteine

Promoter

CMV Dox inducible

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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RCAN1_pCSdest

Plasmid

#53883

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RCAN1 (RCAN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_C_TR61B

Plasmid

#111679

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TR61B (TRMT61B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_ble

Plasmid

#124584

Purpose

Plasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR (cggR Synthetic)

Use

Synthetic Biology

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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N6AMT1_pCSdest

Plasmid

#53843

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

N6AMT1 (N6AMT1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHA8

Plasmid

#187761

Purpose

MAC-tagged gene expression of human EPHA8

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHA8 (EPHA8 Human)

Expression

Mammalian

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPJ174(JPUB_006779)

Plasmid

#83827

Purpose

Carries operon 1 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3603
kuste3605
kuste3606

Use

Pfab217 (p15a ori, kanr)

Promoter

op1_3603_for, op1_3605_for, and op1_3606_for

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Iso C-term GFP

Plasmid

#36130

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 47-204 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Golgi N-term GFP

Plasmid

#36127

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S513A

Plasmid

#175764

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

S513A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CTTN

Plasmid

#67100

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CTTN (CTTN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
TRAPPC10_pCSdest

Plasmid

#53937

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TRAPPC10 (TRAPPC10 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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SIM2_pCSdest

Plasmid

#53790

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SIM2 (SIM2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits