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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF785

Plasmid

#88459

Purpose

Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF785 (ZNF785 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Sc eRF3∆165N (WT)

Plasmid

#175418

Purpose

Encodes His-MBP-TEV-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9-NG

Plasmid

#171692

Purpose

Expresses nSpCas9-NG in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9-NG

Use

CRISPR

Expression

Mammalian

Mutation

D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

Promoter

EF1a

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pB2H2*

Plasmid

#163834

Purpose

Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)

Depositor

Jerome Fox

Insert

SpecR

Use

Synthetic Biology

Expression

Bacterial

Mutation

TCPTP R222M

Promoter

Pro1;ProD;pLacZ-Opt

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF449

Plasmid

#88453

Purpose

Donor Vector containing ZNF449 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF449 (ZNF449 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF770

Plasmid

#88562

Purpose

Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF770 (ZNF770 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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aav-tnt-dnmrtfa-gfp

Plasmid

#165035

Purpose

AAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

dnMRTFA

Use

AAV

Tags

GFP

Mutation

deletion of 100 N-terminal amino acids

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-CARD WT

Plasmid

#164021

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-GSX2-tTA-BleoR

Plasmid

#161748

Purpose

Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Article

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

Advanced tTA

Use

CRISPR

Expression

Mammalian

Promoter

endogenous GSX2 promoter

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

Plasmid

#129513

Purpose

Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 C88S (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA14

Plasmid

#114916

Purpose

Prey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G17230 X074_pECIA14

Plasmid

#114896

Purpose

Prey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G33170 X049_pECIA14

Plasmid

#114901

Purpose

Prey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA14

Plasmid

#114880

Purpose

Prey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G02880 J3_pECIA14

Plasmid

#114790

Purpose

Prey vector AT3G02880 J3_pECIA14 should be used with bait vector AT3G02880 J3_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02880 (AT3G02880 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G51890 M5_pECIA14

Plasmid

#114744

Purpose

Prey vector AT1G51890 M5_pECIA14 should be used with bait vector AT1G51890 M5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G51890 (AT1G51890 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G66830 X005_pECIA14

Plasmid

#114810

Purpose

Prey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G66830 (AT1G66830 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS663-pMCSG7-AcrIIC4Hpa

Plasmid

#113440

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Tags

6xHis

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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