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Addgene

We narrowed to 12,754 results for: CAR

Showing: 11921 - 11940 of 12754 results
  1. phCMV-coGALVenv-C4070A

    Plasmid
    #172217
    Purpose
    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors
    Depositor
    Insert
    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Carries C-terminus of amphotropic MLV strain 4070…
    Promoter
    CMV immediate early promoter
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid
    #218207
    Purpose
    Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Insert
    -2.1prox1a_1
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

    Plasmid
    #184463
    Purpose
    pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.
    Depositor
    Insert
    N-Flag-mSTAT5bCA (Stat5b Mouse)
    Use
    AAV
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Point mutation at base 1963 (A to C), which chang…
    Promoter
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. SnapTag-Binder

    Plasmid
    #179142
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Insert
    SspB
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. Flag-mCherry-Binder

    Plasmid
    #179139
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Insert
    SspB
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Spectinomycin resistance gene promoter and no
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Tags
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 11921 - 11940 of 12754 results