We narrowed to 92,229 results for: lias

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pSAVED-CHAT-sm_PCaspase

Plasmid

#214048

Purpose

Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT

Plasmid

#214030

Purpose

Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-dSAVED-CHAT

Plasmid

#214031

Purpose

Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Tags

Strep-tag II

Expression

Bacterial

Mutation

H375A, C422A

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT-sm

Plasmid

#214033

Purpose

Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Tags

Strep-tag II

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase

Plasmid

#214034

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

Plasmid

#199242

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S191C, I225A, L228A, W231A

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4B(S184C)

Plasmid

#180026

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S184C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRgp78

Plasmid

#185344

Purpose

Expression of soluble G2BR, amino acids 575-600 of human gp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 aa 574-600 (G2BR) (AMFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 575-600 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-RBMS3

Plasmid

#185557

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

RBMS3 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-HHIP

Plasmid

#185551

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

HHIP gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

Plasmid

#185554

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

ADGRG6 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G, T805C, C813S)-Strep

Plasmid

#169749

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine, Threonine 805 to Cysteine…

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G, G808C, C813S)-Strep

Plasmid

#169750

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine, Glycine 808 to Cysteine, …

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Slc1a3 nickase 1

Plasmid

#129410

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, S712C, P801G, C813S)-Strep

Plasmid

#164445

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Serine 712 to Cysteine, Proline 801 to Glycine, C…

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, A756C, P801G, C813S)-Strep

Plasmid

#164446

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Alanine 756 to Cysteine, Proline 801 to Glycine, …

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G, C813S, S863C)-Strep

Plasmid

#164447

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine, Cysteine 813 to Serine, S…

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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