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pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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helicase cDNA

Plasmid

#39552

Depositor

Norbert Perrimon

Article

Eberl et al Genetics. 1997 Jul;146(3):951-63.

Insert

helicase (Hel25E Fly)

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 xBicaudal-C

Plasmid

#24959

Depositor

Edward De Robertis

Article

Wessely et al Development. 2000 May . 127(10):2053-62.

Insert

Bicaudal-C (bicc1-b Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pColdI-DDX3_helicase_core_Q360L

Plasmid

#233619

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Leu (DDX3X Human)

Tags

His6

Expression

Bacterial

Mutation

Gln360Leu

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360P

Plasmid

#233620

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Pro (DDX3X Human)

Tags

His6

Expression

Bacterial

Mutation

Gln360Pro

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-49-KanR

Plasmid

#203534

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI49 Cap

Use

AAV

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Silene_conica _GlnRS_MitoTP_pK7FWG2

Plasmid

#202654

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _LysRS_MitoTP_pK7FWG2

Plasmid

#202655

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _TyrRS_MitoTP_pK7FWG2

Plasmid

#202657

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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